Aerobic granulation of single culture protist

Thraustochytrium striatum was selected as a model protist to test the possibility of granulation for its good potential organic waste valorization. Initially, only selective settling velocity 9 m h−1 applied in sequential batch reactor (SBR) with minimal progress after three months. After famine condition introduced into SBR feast-to-famine duration ratio 0.2 by extending cycle time and lowering initial substrate concentration while keeping unchanged, granules spherical shape smooth surface formed were dominated another month. The average particle size 728 μm, 90 times greater than planktonic counterpart. This study evidenced culture-independent phenomenon aerobic granulation. Also, combination low found be required inducing successful